The Ren and Stimpy "The Last Temptation" Collection consists of animation production drawings, exposure sheets, and overlay/underlay drawings used to produce "The Last Temptation" episode. The Ren and Stimpy Show was an animated television series created by Canadian animator John Kricfalusi. It focuses on the antics and adventures of Ren, a temperamental Chihuahua, and Stimpy, an excessively happy cat. The show premiered on Nickelodeon, an American cable and satellite television network, on August 11, 1991. It ran for five seasons, and was produced by Kricfalusi's animation studio, Spümcø, for the first two. Beginning with season three (1993–94), the show was produced by Nickelodeon's Games Animation, which later became the Nickelodeon Animation Studio.

The show ended its original run on December 16, 1995, though "The Last Temptation" did not air in the series' original run. Nickelodeon's sister network, MTV, aired the episode on October 20, 1996. The story was developed by Jim Gomez, Vince Calandra, and Bob Camp, who also directed the episode. The collection is arranged by scene number.